Журнал «Агропромышленные технологии Центральной России»
Выпуск №4 (22) (2021)
УДК 581.16: 635-153
DOI 10.24888/2541-7835-2021-22-57-67
ВЛИЯНИЕ КРАТКОВРЕМЕННОГО ТЕМПЕРАТУРНОГО СТРЕССА НА ПРОРАСТАНИЕ СЕМЯН УКРОПА
Получены новые данные о процессе прорастания семян укропа первого и второго порядков после кратковременного теплового стресса (40°С). Проращивание семян проводили в термостате. Набухшие семена (четыре повторности по 100 семян) подвергали воздействию температуры 40°С в соответствии со схемой опыта: 0 (контроль); 1; 2; 3; 4 и 5 суток. После инкубации семена переносили в стандартные (t = 20°С) условия и проращивали в чашках Петри на фильтровальной бумаге без доступа света в течение 21 суток. Использованы методы анализа прорастания семян в динамике. Для построения кривой прорастания семян использовали лог-логистическую регрессию с тремя параметрами: b, d, e. Все статистические анализы были выполнены в R версии 3.4.3. Выявлена продолжительность действия высокой температуры, которая оказывает ингибирующее действие на скорость прорастания и количество проросших семян. Семена первого порядка в контроле и после инкубации в течение 1-3 суток прорастали сходным образом, а при увеличении периода инкубации до 4-5 суток темпы прорастания резко снижались. Семена второго порядка отличались еще меньшей устойчивостью к действию короткого высокотемпературного стрессора, и при инкубации семян в течение 3 суток и последующем переносе их в стандартные температурные условия не прорастали. Время прорастания 50% семян (Т50) первого порядка в результате последовательного увеличения периода инкубации в условиях 40°С увеличилось от 0,92±0,11 до 6,4±0,49 (р
THE EFFECT OF SHORT-TERM TEMPERATURE STRESS ON THE GERMINATION OF DILL SEEDS
New data on the process of germination of dill seeds of the first and second orders after short-term heat stress (40°C) have been obtained. Seed germination was carried out in a thermostat. Swollen seeds (four repetitions of 100 seeds) were exposed to a temperature of 40° C in accordance with the scheme of the experiment: 0 (control); 1; 2; 3; 4 and 5 days. After incubation, the seeds were transferred to standard (t = 20° C) conditions and germinated in Petri dishes on filter paper without access to light for 21 days. Methods of analysis of seed germination in dynamics are used. Log-logistic regression with three parameters was used to construct the seed germination curve: b, d, e. All statistical analyses were performed in R version 3.4.3. The duration of the action of high temperature, which has an inhibitory effect on the germination rate and the number of germinated seeds, was revealed. Seeds of the first order in the control and after incubation for 1-3 days germinated in a similar way, and with an increase in the incubation period to 4-5 days, the rate of germination decreased sharply. The seeds of the second order were even less resistant to the action of a short high-temperature stressor, and when the seeds were incubated for 3 days and then transferred to standard temperature conditions, germination did not occur. The germination time of 50% of seeds (T50) of the first order as a result of a sequential increase in the incubation period at 40° C increased from 0.92 ± 0.11 to 6.4 ± 0.49 (p
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